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coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)

doi: 10.1111/j.1463-1326.2012.01557.x 159 AnvariMPatersonCADanielEEMcDonaldTJ

When it shows up, it's mild and dose-dependent

It also helps you notice when youre actually full

Here are a few reasons your eye color may appear different versus the factors that may cause it to change