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Cell viability, LDH content and intracellular ROS assays After seeding the cells in the 96-well plate for 24 h post-transfection, they were treated with AFB 1 at the concentration of 10, 25, 50, 100 and 200 g/L

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We also discuss potential therapeutic targets, such as Transient Receptor Potential Vanilloid 1 (TRPV1), Glutathione Peroxidase 4 (GPX4), and Nuclear Factor Erythroid 2-Related Factor 2 (NRF2), which modulate ferroptosis and OA progression

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