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Animals A total of 90 adult male Wistar rats, weighing approximately 200 to 250 g, were purchased from the Animal Experiment Center of the Chinese Academy of Medical Sciences, Beijing, China and were maintained in wooden cages at a temperature of 27 C30 C, with a 12 h light-dark photo-periodicity cycle

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However, basic research suggests that its aberrant expression may compromise mucosal barrier integrity, acting as a potential susceptibility factor for disease, although longitudinal studies clarifying the temporal relationship are lacking