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Analysis and visualization of PCA and differentially expressed proteomic data Raw data files were processed using the DIA-NN software v.1.8.1 (DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput) [31] available on GitHub ( DIA-NN was installed in an Apptainer container using the Docker image from Docker Hub ( analysis was conducted with default parameters, allowing for up to two permissive cleavages and protein N-terminal methionine as a variable in the in-silico digestion

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