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All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction

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The study supports the utility of DSIP-CBBBP in developing therapies targeting neurotransmitter dysregulation and sleep disturbances, offering a promising avenue for future research in sleep-related treatments

Based on sequence similarities in particular in the regions outside the forkhead box domain, the sub-families have been classified from FOXA to FOXR with at least 41 genes currently identified in humans [5]