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A first-in-human, open-label phase 1b and a randomised, double-blind phase 2a clinical trial in recent-onset type 1 diabetes with AG019 as monotherapy and in combination with teplizumab

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doi:10.1007/82_2011_189

The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)