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Of the 3 pellets, 2 were resuspended in 40 L of lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1% Triton, 1 PIC), while the third pellet was resuspended in 1 glyco-buffer 2 (50 mM sodium phosphate pH 7.5, 1% NP-40, 1 PIC)

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Online calculators exist from the CDC, NIH, and countless medical websites