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Nature 569 , 641648 (2019)
The tissue was then incubated in Hanks buffered salt solution (HBSS) containing 15 mM HEPES, 50 U/mL collagenase type II (Thermo Fisher 17101015), and 100 U/mL hyaluronidase (Sigma Aldrich H3506) at 37 C for 30 min
Certain Bifidobacterium and Lactobacillus species can produce GABA 296,297,298,299,300 , and oral treatment with GABA or bacteria that produce GABA improves metabolic parameters in mice 298,301