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Cryo-EM data acquisition 3.5 L of purified hGLP-1:rGLP-1R:Gs complex at a concentration of ~1 mg/ml complex was applied to glow-discharged holey carbon grids (Quantifoil R2/2, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (FEI Company)

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Although semaglutide causes the stomach to empty more slowly, leading to gastrointestinal symptoms like bloating, no significant evidence shows that it increases the risk of colon perforation