For G protein and -arrestin2 receptor recruitment studies, we used human G i1 with nanoluciferase (NanoLuc) inserted at position 91 (NanoLuc-G i1(91) ) and human -arrestin2 fused with NanoLuc at the N terminus (NanoLuc--arrestin2)
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Analogue 1.70 The analysis of Compound 1.70 yielded the following results: ESI-MS was used to confirm a [M + H + ] ion at m / z 321.0, which is indicative of the expected molecular mass
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