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Library Preparation and Sequencing (1) Amplicon Purification PCR products ( 550 bp) were size-verified by 2% agarose gel electrophoresis and purified (TIANgel Midi Purification Kit, Tiangen, DP209, China)

Combining the two can intensify this effect

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This approach aligns with the goals of a systematic review into a novel research area, where the primary aim is to gather and describe existing knowledge rather than to rigorously assess study quality or synthesize outcomes quantitatively