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For pooled analysis of results from different independent experiments, all participants/mice from the same experimental group were pooled, and a new statistical comparison was made for the entire pooled experiment, as performed for the individual experiments

IGF-1 is a naturally produced polypeptide amino acid

A second aliquot of 10 l ghosts was solubilized for 1 h at room temperature in 1 ml of PBS containing 3 mM EDTA and 0.05% (vol/vol) Triton X100 and used to quantify hemoglobin

An in vivo aging detection platform, such as QM--gal, has been established to detect senescent cancer cells

During adolescence, attention-seeking often intensifies