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Chemogenetic activation of GLP-1 neurons in NTS similarly decreased nicotine intake

More specifically, the researchers posited that the peptide may have enhanced the expression of Ki-67 and CD98hc that are less intensively synthesized during cell aging

Cells resuspended in the growth medium (GM, DMEM containing 10% fetal bovine serum (FBS, Life Technologies) and 1% anti-anti), and the single cells were seeded in a cell culture flask

PubMed Functional and Aesthetic Periorbital Changes Linked to GLP-1 RAs

Cells were lysed in 1 mL ChIP lysis buffer (50 mmol/L HEPES-KOH, pH 7.5, 140 mmol/L NaCl, 1 mmol/L EDTA, pH 8.0, 1% Triton X-100, and 0.1% deoxycholate with 0.1 mmol/L PMSF and the EDTA-free protease inhibitor cocktail)