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In beta cells, WFS1 deficiency impairs insulin synthesis and secretion and induces apoptosis [4, 6, 7]

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Diethyl ether (1 mL) was added for precipitation, the mixture was centrifuged, the supernatant discarded, and the peptide pellet washed with diethyl ether (35) by centrifugation

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Additionally, the appetite-suppressing effects of these medications often lead to reduced food and fluid intake, which can further contribute to constipation if adequate fibre and fluids aren't maintained