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The homogenate was then centrifuged at 13,000 rpm at 4 C for 15 min

Reconstituted solutions should be refrigerated at 28C

The intestinal microbiota of adolescents appears to be in an intermediate stage of development in terms of taxonomic composition, as shown by the high relative abundance of bacteria such as Bifidobacterium and Bacteroides fragilis , which are highly abundant in infants and toddlers (Hopkins et al., 2001