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Gene expression analysis with RTqPCR Gene expression was analyzed via reverse transcriptase quantitative polymerase chain reaction (RTqPCR) using TaqMan probes (Life Technologies) or SSO Universal Probes Master Mix (Bio-Rad)

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Its ability to stabilize natural sleep patterns could reduce dependency on traditional sedatives or hypnotics, which often carry unwanted side effects

Including all doses in the charts above would have made them unreadable so we eliminated similar dose-responses