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Transamination Reactions: Glutamate can also be converted to -KG through transamination with aspartate by the action of aminotransferases, which transfers the amino group to oxaloacetate, producing -KG

The proteins were purified from cell culture supernatant using a human albumin affinity column, followed by size exclusion chromatography (SEC), which yielded monomeric fractions that migrated on non-reducing sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels according to their molecular weight (MW) (66.5 kDa) (Fig