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Both cells and islets were stimulated with 100 nM exendin-4 or vehicle for 10 minutes and subsequently labelled with MitoTracker Red CMXRos (Thermo Fisher Scientific), washed 1 with PBS and imaged in phenol red free RPMI-1640 in a Leica Stellaris 8 inverted confocal microscope with 63/1.40 NA (islets) or 100/1.40 NA (cells) oil immersion objectives