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To quantify protein levels in CD8 + T cells, single-cell suspensions were stained using the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Thermo Fisher Scientific, 10119), followed by surface and intracellular staining with fluorochrome-labeled antibodies (Supplementary Table 1)

Patients with uncontrolled tachyarrhythmias, recent acute coronary syndrome, or unstable heart conditions should exercise caution and require thorough cardiovascular assessment before starting treatment

The combination is well-established in clinical guidelines and considered a standard step-up approach when metformin alone does not achieve target glucose levels

Harper et al

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