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4.Enzyme marker inactivation or substrate failureMix the enzyme conjugate and substrate and check and judge by rapid color development Problem description: Low readingsPossible causes and corresponding countermeasures: 1.The microplate reader is not set up correctlyCheck wavelength and filter settings on the microplate reader/Turn on the ELISA reader in advance to preheat Problem description: Large coefficient of variationPossible causes and corresponding countermeasures: 1.Incorrect addition of liquidCheck the filling status Problem description: High background valuePossible causes and corresponding countermeasures: 1.The working concentration of the detection antibody is too highUse the recommended dilution factor 2.Incomplete washing of ELISA plateEnsure that each step is cleaned completely

The RNA sequencing data presented in this article were deposited in the Gene Expression Omnibus (GEO) database under accessions GSE283949 and GSE277651

The post says centres generally prefer donors to have been on a stable medication regimen for at least 3 to 6 months

Raw data were normalized and analyzed using GeneSpring software (Agilent Technologies)