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It must also be completely free of solutes, meaning there are no added buffers or minerals that could alter the chemical behavior of your test peptide

Monitor it, note when it occurs and how long it lasts, but mild post-injection warmth doesn't require intervention

You might be using a solution you think is at a specific concentration, but in reality, it could be 50% or less of its original strength

doi: 10.1111/j.1476-5381.2011.01687.x

2.4 Bioluminescence resonance energy transfer assays To generate dose-response curves, HEK-293 cells were transiently transfected with equal amounts of labeled receptors and labeled G protein for G protein recruitment assay and in a 1:2 ratio for arrestin and KRAS assays