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Elkanzi NAA, Hrichi H, Alolayan RA et al (2022) Synthesis of chalcones derivatives and their biological activities: a review

Arg, Ala, Glu and Tyr were selected as replacements to distinguish the relevance of the charge and size of the side chain and to mimic tyrosine-dependent glutathione transferases 3,18

Li Y, Zhang L, Zhang Q, Zhang Y, Pan S, Zhao H, et al

The final measurement at the end of this step reflects the total content of GSH (free GSH + GSSG) in the samples, to calculate the GSSG content, the free GSH content must be subtracted from the total GSH content

Furthermore, the activation of AMPK by berberine directly phosphorylates and activates endothelial nitric oxide synthase (eNOS), the enzyme responsible for producing the vasodilating molecule nitric oxide (NO)