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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 mM L-glutamine, and 5mM cytosme-D-arabinofuranoside from Sigma added at 2 days in vitro

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In response to hepatocyte injury, Kupffer cells release CCL2, facilitating monocyte recruitment and migration to the liver, where they differentiate into pro-inflammatory macrophages [58]

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Similarly, high plasma levels of free fatty acids have been shown to stimulate splanchnic somatostatin, thereby affecting GH secretion (419)