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Sections containing ARH were washed with PBS three times for 5 minutes each and treated with a blocking solution (3.0% goat serum, 0.3% triton-X, and filtered PBS) for 1 hour at room temperature

Studies have demonstrated that lowering HbA1c significantly reduces the risk of these complications, highlighting the importance of strict glycemic control

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To induce acute liver injury, 8-week-old NSI mice received two consecutive intraperitoneal injections of DMN (7 mg/kg, Sigma, 1.0% dissolved in saline)