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Furthermore, serum IL-6 and lipopolysaccharide-binding protein levels were significantly reduced and butyric acid/isobutyric acid was significantly increased in patients with AUD-related cirrhosis who received FMT compared with placebo

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The membrane was blocked with 5% milk in TBST (20 mM Tris-HCl pH 8.0, 150 mM NaCl and 0.05% Tween-20), then incubated with the anti-GluK2 monoclonal antibody (2000-fold dilution, 04-921, Sigma-Aldrich), followed by horseradis peroxidase (HRP)-conjugated anti-rabbit antibody (10,000-fold dilution, ab97051, abcam)