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The universal primers F: 5- ACTCCTACGGGAGGCAGCA-3 and R: 5- GGACTACHVGGGTWTCTAAT-3 were employed for PCR amplification of the bacterial 16S rRNA gene 16S_V3V4a region, and the universal primers F: 5- GGAAGTAAAAGTCGTAACAAGG-3 and R: 5- GCTGCGTTCTTCATCGATGC-3 were used for PCR amplification of the fungal ITS gene ITS_V1 region

Implementing stress-reduction techniques like meditation, yoga, deep breathing, adequate sleep, and light to moderate exercise helps support healthy histamine levels

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