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ALIs were harvested for wholemount IF labeling on day 16

Indeed, within the first 4 days of quitting, initial caffeine clearance decreased significantly by 36% (Faber and Fuhr, 2004)

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Plates were incubated in a constant-temperature incubator at 28 C for 3 d

Recent studies have shown that early, repeated intake of nicotine is associated with cognitive decline and performance impairment