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As the number of chewing tobacco users continues to increase in many low- and middle-income countries, our findings reflect the importance of better incorporating chewing tobacco into broader tobacco control efforts that have been instrumental in reducing the burden of smoked tobacco (Table 1)

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Then the membrane was incubated with anti-rabbit polyclonal antibody (1:1000) for overnight at 4 C, followed by incubation with alkaline conjugated anti rabbit antibody (1:2500) for 2.0 h

this represents the most recent time period since the last systematic review of the available evidence for the GBD at the time of publication