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Total RNAs were extracted from transfected protoplasts with TRIzol reagent at 24 h, 48 h and 72 h, respectively, followed by cDNA synthesis with primer R using the SuperScript III Reverse Transcriptase kit (Invitrogen)

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By securing permanent protection through a conservation easement, the Tobacco Road Mitigation Project will help improve water quality, enhance biodiversity, and contribute to regional conservation efforts