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The aqueous phase was made alkaline with 1 mL 1M NaOH, 2M KCl and extracted again with 4 mL CH 2 Cl 2 and 4 mL CH 2 Cl 2 :iPrOH (75:25) consecutively

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Hindsight shows us that after Season 5 Reed again considered ending the series, reportedly expressing an opinion that the writers had run out of fresh ideas and wanting to spend more time with her family, but somehow she and Owen agreed to another 3-year contract before finally calling it quits in 1966, again citing that she was tired of the production requirements