The evaluation of procollagen I synthesis, and IL-6 and TIMP-1 production were assessed by ELISA with the Human Pro-Collagen I alpha 1 ELISA kit (#ab210966, Abcam, Waltham, MA, USA), the Human IL-6 ELISA kit (#ab178013, Abcam, Waltham, MA, USA) and the Human TIMP1 ELISA kit (#ab187394, Abcam, Waltham, MA, USA), while MMP-1 activity was evaluated with the Human Active MMP-1 Fluorokine E Kit (#F1M00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions
Corts-Rojas DF, de Souza CR, Oliveira WP
Walker, K., Ramstein, J., & Smith, J
"Fact Sheets -- Underage Drinking." Nov
Furneaux delivers the reading without looking up from her notes, making it harder for listeners to connect, and her rhythmic incantations make it difficult to actually grasp the meaning of her words