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Paul Thomas Anderson used this very setting to begin his career with Cigarettes and Coffee below

Following three 5 min washes in PBS-T, membranes were incubated with Chemiluminescent Substrate (Thermo Scientific, 34580), and bands were visualized using Autoradiography Film (Denville Scientific, E3012)

He explained that he intended to depict how much damage smoking a pack of cigarettes for 30 days straight - which equates to 600 individual ones - could have on you

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