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After being incubated for 24 h, the culture media of the cells were removed and replaced by 2 mL EGM-2 per well in the control group, and by 2 mL 1%CSE solution per well in the CSE group and CSE+C646 group, 2 mL CTPB solution per well in the CTPB group (final concentration of CTPB was 25 M)

This results in a lighter smoking experience in terms of the amount of tobacco consumed per cigarette

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Since these products are new, the long-term health effects of using heated tobacco products is not yet fully known