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In this study, we used a G protein-coupled receptor-activated DA fluorescent probe (GRABDA1m) and optical fiber photometric recording equipment to monitor the dynamic changes and kinetics of DA release in the NAc of mice exposed to different e-cigarette aerosols, including nicotine, nicotine benzoate, nicotine tartrate, nicotine lactate, nicotine levulinic acid, nicotine malate, and nicotine citrate

For this, drug molecule or the metabolite becomes a hapten that binds with protein to form an antigen which then is processed by APCs

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Exemptions: Local regulations enacted before December 31, 2020, remain valid