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As for the blank solution, 2.5 mL of unitary phosphate buffer (PB) solution was used, while a solution mixture (1.45 mL of PB + 50 L of H 2 O + 1 mL of diluted H 2 O 2 ) was taken as a reference analyte, and in the test sample (1.45 mL of PB + 50 L of plasma sample + 1 mL of diluted H 2 O 2 ) was used

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The PAF was calculated by the following Levins formula for multiple categories (k), as proposed by Hanley [62, 63]: where RR is the relative risk of cancer for smoking, p is the smoking prevalence in the total adult population (aged 20+ years), and K is the number of categories in the smoking exposure