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It is important to note that the major IgE epitopes of polcalcins are not located in conserved calcium-binding regions (Ricciardi et al., 2022), but calcium binding to EF-hand motifs leads to conformational changes in protein structures and affects stability and IgE-binding capacity of the allergens of this class (Parody et al., 2013)

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[3] Anderson expanded the film into his first feature film, Hard Eight (1996)

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