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While people of all ages around the world enjoy coffee, nicotine has been heavily stigmatized. But nicotine and caffeine arent chemically similar, not really

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A seven-point calibration curve was constructed with nicotine concentrations of 10, 25, 50, 100, 250, 500, and 1,000 ng/mL with 250 ng/mL of nicotine-d4 as internal standard