Gm G And thinkin' that I was gonna die before thirty, G your mom was so worried about F Gm All those kids in the house tearin' picture frames down G F Gm G Our fathers were never around when we were younger [CHORUS] F G So let me go, I saw you on the river's edge G C Draggin' on a plastic cigarette C Gm With your swim top still wet F G So let it go, meetin' you out on the coast G C You hate the smell of real smoke C Gm So why'd you always keep me so close
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The only Kardashian sister not in attendance was Kourtney Kardashian
At 80% flask confluency, cells were harvested using DPBS for washing and incubated with 1.5 ml of 0.25% trypsin EDTA/DPBS and poly-vinyl-pyrrolidone for 34 mins at 37C to allow detachment