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Lentiviral transduction by VectorBuilder (Chicago, USA), SigmaAldrich (MO, USA) and SCBT (Santa Cruz Biotechnology, CA, USA) was performed as previously described [19] The transfection efficiency of the shRNAs was evaluated by Western blotting

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Paramedics received a 911 call at 12:21 pm Pacific time (19:21 UTC) and arrived four minutes later