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Total reaction volume was 20 L, containing 5 ng DNA and 75 M of forward and backward primers, F015 5-CAC CCT ATT AAC CAC TCA CG-3 and R569 5-GGT GTC TTT GGG GTT TGG TTG-3, respectively

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In 2024, a total of 47.4% of youth who used e-cigarettes had considered quitting in the past month, while 25.3% had attempted to quit in the past year 1