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In previous studies, AlkB has been reported to efficiently remove methylations in N 1 -methyladenosine (m 1 A), N 3 -methylcytosine (m 3 C), and the AlkB(D135S) variant can efficiently demethylate N 1 -methylguanosine (m 1 G)

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It basically relies on an arrest-and-release strategy using aphidicolin, an inhibitor of DNA replication, and propyzamide, a plant-microtubule disruptant

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