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As a result, there is reduced concentration of these antipsychotics in medicated SZ smokers, which predictably leads to a reduction in side effects, motivating further use (113) (supporting the self-medication hypothesis)

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Real time quantitative PCR analysis Real-time quantitative PCR amplification was conducted by pipetting 4.4 L of cDNA (diluted 1:25 or 1:50 in MilliQ water), 5 L 2xSensiMix SYBR and Fluorescein Kit (Bioline, the Netherlands) and 0.6 L forward and reverse primers for target genes in white LightCycler480 384 multiwell plates (Roche)

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