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After centrifugation at 400 g for 6 min at 4 C, supernatants (cell-free BAL fluid) from the first two aliquots of 0.5 ml were stored at 80 C for cytokine analysis (ELISA), and cell pellets were used for flow-cytometry analysis

Some people subjectively feel that temperature control coils alter the flavor of e-cigarette vapor slightly

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[ 38 ] A study of Scottish youth found that those who recalled seeing e-cigarettes in retail settings were nearly twice as likely to have tried an e-cigarette, and also more likely to report intentions to try them within the next six months

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