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The eluted protein was concentrated using an Amicon 100 kDa MWCO Ultra spin column (Millipore) and then subjected to size-exclusion chromatography using a Superose 6 increase 10/300 GL column (Cytiva) equilibrated with 1xTBS, 0.02% GDN

Groups at higher risk of deficiency include older adults (due to reduced stomach acid production), vegans and vegetarians, people with pernicious anaemia or gastrointestinal disorders affecting absorption, and those taking certain medications such as metformin or proton pump inhibitors

315 , R323R335 (2018)

It is a freeze-dried powder, a white to off-white cake of lyophilized semaglutide sitting at the bottom of the vial