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The gray and white matter variables utilized in this study were derived from the image-derived phenotypes (IDPs) released by the UK Biobank team (for more details on the processing pipeline and IDP generation for the gray and white matter measures see [34])

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Whereas we operationalized chronic effects as structural alterations identified in smoker versus nonsmoker (i.e., between-subjects) comparisons, we operationalized the acute effects of nicotinic acetylcholine receptor (nAChR) agonist administration as functional alterations indentified in pharmacological neuroimaging studies, the vast majority of which employed within-subjects (i.e., drug versus control condition) comparisons

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Then an anesthetized rat is exposed to the smoke for one minute