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Individuals who smoke even half a pack of cigarettes a day may [be at] increase[d] risk of having [peripheral arterial disease] by up to 50 percent. However, it is important to note that up to 40 percent of peripheral arterial disease patients are asymptomatic, often leaving the condition misdiagnosed

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Year: 2007

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For detecting ROS by IF, slides were incubated with dihydroethidium (DHE) solution (Sigma, D7008) at 37 C for 30 min, succeeded by counterstaining with DAPI (Beyotime, C1002) for 10 min at room temperature in the dark