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Mobile phase A consisted of 0.01% NH4OH in water and mobile phase B of 0.01% NH4OH in methanol with a gradient starting at 5% mobile phase B which linearly increased to 90% B at 2 min and to 100% B at 2.5 min

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Monitor Your Nicotine Intake If you are exceeding 600 puffs daily, consider adjusting your nicotine strength