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Briefly, hASM cells plated in eight-well chambered coverslips (Ibidi, Grafelfing, DEU) were incubated in Hanks balanced salt solution (HBSS) containing 1 M Rhod 2-AM (Thermo Fisher Scientific, Waltham, MA) for 45 min at room temperature, medium aspirated, and washed three times in HBSS and incubated further for 15 min to allow for deesterification of the Rhod 2

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