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The total RNA (4 L) from each sample was used for reverse transcription using a reverse-transcription reagent kit (PCR005

DISCUSSION Our results showed that major reasons for not trying e-cigarettes among Chinese adult smokers included low intention to quit smoking, concerns about e-cigarettes effectiveness as a smoking cessation method, and belief of being able to quit any time without aid, as well as concerns about substitution with another addictive behavior, concerns about e-cigarettes safety, appearance, and cost

This is very much a work in progress

PMID 12390575