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Comparative analysis of the activities of recombinant TEV, ACP-TEV and TEV-R9 For comparative analysis of the activities of recombinant TEV and its ACP-TEV fusion protein, the substrate MBP-BCCP was mixed with TEV and ACP-TEV at a molar ratio of 10:1, and incubated at 25 C in standard reaction buffer (50 mM TRISHCl, 0.5 mM EDTA, 1 mM DDT, pH 8.0)